Photo-induced conjugation of tetrazoles to modified and native proteins.

نویسندگان

  • Winna Siti
  • Amit Kumar Khan
  • Hans-Peter M de Hoog
  • Bo Liedberg
  • Madhavan Nallani
چکیده

Bio-orthogonal chemistry has been widely used for conjugation of polymer molecules to proteins. Here, we demonstrate the conjugation of polyethylene glycol (PEG) to bovine beta-lactoglobulin (BLG) by photo-induced cyclo-addition of tetrazole-appended PEG and allyl-modified BLG. During the course of the investigation, a significant side-reaction was found to occur for the conjugation of PEG-tetrazole to native BLG. Further exploration of the underlying chemistry reveals that the presence of a tryptophan residue is sufficient for conjugation of tetrazole-modified molecules.

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عنوان ژورنال:
  • Organic & biomolecular chemistry

دوره 13 11  شماره 

صفحات  -

تاریخ انتشار 2015